FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282367

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282367
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211359
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC72983.4528929451785824No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC52152.4673659508230075No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC34051.6110030800675628No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC24981.1818753873740886No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA7760.3671478385117265No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT6130.2900278672779489No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG6000.2838771947255617No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA5350.25312383196362587No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG4180.19776777899214132No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTCTT4020.19019772046612637No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGC3930.1859395625452429No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTTT3880.18357391925586325No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTAAA3690.17458447475622044No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCC3460.16370251562507393No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAAAG3430.16228312965144612No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTTTG3380.15991748636206643No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGA3250.15376681380967927No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGATG3220.15234742783605146No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATGG3170.14998178454667177No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATTC3170.14998178454667177No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3120.1476161412572921No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGTAAA3100.1466698839415402No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATG2960.1400460827312771No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATG2940.13909982541552524No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG2910.13768043944189745No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT2860.13531479615251774No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTG2840.1343685388367659No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG2800.13247602420526214No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTC2750.13011038091588245No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTTTC2660.125852222994999No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGCTTC2570.1215940650741156No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGACTG2540.12017467910048779No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCAATC2510.11875529312685999No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCCGG2470.11686277849535624No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTGT2450.11591652117960438No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGAACTC2410.11402400654810063No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC2350.11118523460084502No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGCTTT2130.10077640412757441No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG13000.0117.659151
GTCTCTC17850.0113.1488342
AGCTGGT18150.0111.464669
CTCAGCT18450.0109.652236
TCAGCTG18800.0107.610847
TCTCTCA19000.0107.034263
AGTCTCT18950.0106.225361
CAGCTGG19100.0105.92068
CTCTCAG19250.0105.569154
TCTCAGC20800.097.263645
CTCAAAC20650.074.262079
GGCTCAA21050.072.850927
GCTCAAA21200.072.335468
GATGGCT21950.070.914974
TGGCTCA21800.070.6748356
CTGATGG22350.069.3890842
ATGGCTC23700.064.705145
TGATGGC25500.061.0858883
TCAAACA21550.054.790359
GCGACAA200.006285250728.798342120-124
Produced by FastQC (version 0.11.5)