FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282369

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282369
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201620
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTACG400.0144.153961
AACGTAC100.007238568143.97525
AATCCGC100.007238568143.97526
TTAGCTA100.007238568143.97526
CGTGCGA100.007238568143.97527
GCGCGTC100.007238568143.97524
AACGGGC100.007238568143.97522
CGAGAAT100.007238568143.97522
ATACGAG100.007238568143.97523
GTACGAA100.007238568143.97528
GTCGTTA151.2637663E-4143.975195
CCTATAG151.2637663E-4143.975193
TCTATCG151.2637663E-4143.975198
TAGTTGG550.0131.049061
ATCCGAA500.0129.738561
AGACGAC450.0127.977958
TCGCGAA403.45608E-11125.9782949
GCGCGAT351.9736035E-9123.560541
AATCGAA351.9899744E-9123.407329
TGACTAG950.0121.392811
Produced by FastQC (version 0.11.5)