FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282370

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282370
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194248
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGTA400.0143.99821
CGATTGA253.8564394E-8143.998181
TAGTCCG351.2732926E-11143.961145
CTAGACG100.0072404807143.961126
TCCGATA100.0072404807143.961128
TAGACGA100.0072404807143.961127
TGGTTCG202.2037093E-6143.961124
GTATGAC202.2037093E-6143.961129
ACGTCGA100.0072404807143.961126
CCGAATG202.2037093E-6143.961129
CGGTCGA100.0072404807143.961126
CGCGTAC100.0072404807143.961125
TGTCGAG100.0072404807143.961127
GTCGAAC151.2641877E-4143.96118
GGCTCGA600.0131.998341
TCGTGAC500.0129.5658
CATATTC450.0127.99841
TAAACCG403.45608E-11125.998431
GTCGACT403.45608E-11125.965988
CCTTCGA750.0124.798441
Produced by FastQC (version 0.11.5)