FastQCFastQC Report
Tue 7 May 2024
EGAF00005282375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203544
Total Bases1.6 Mbp
Sequences flagged as poor quality0
Sequence length8
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCTTGA20072598.61504146523602No Hit
GATCTTAA3900.1916047635891994No Hit
GATCTAGA2780.13657980584050625ABI Dynabead EcoP Oligo (100% over 8bp)
GATCTCGA2620.12871909759069292No Hit
GATCTTGC2540.12478874346578626No Hit
GATGTTGA2500.12282356640333295No Hit
GATCTTGG2500.12282356640333295No Hit
GATCGTGA2390.11741932948158629Illumina PCR Primer Index 1 (100% over 8bp)

[WARN]Adapter Content

Can't analyse adapters as read length is too short (12 vs 0)

Produced by FastQC (version 0.12.1)