FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282376

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282376
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203544
Sequences flagged as poor quality0
Sequence length150
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGTA100.007241353143.9574
GCCGATA151.2644201E-4143.9572
TGCGACA100.007241353143.9573
TCGCTAG202.2042805E-6143.9578
TCAGACG151.2644201E-4143.9577
CGGGATA151.2644201E-4143.9572
AACTGAG151.2644201E-4143.9573
TCGTAGC100.007241353143.9576
GATTGAC202.2042805E-6143.9572
TCGCGAT100.007241353143.9578
CGATTGA600.0131.960571
CGCTATC550.0130.871
TCGATAG403.45608E-11125.962388
AGCGAAC351.9917934E-9123.391719
TACGTTA351.9917934E-9123.391715
TACGGTA650.0121.809761
GGTCGAA301.1468546E-7119.9641657
AGACGAC301.1468546E-7119.9641658
TTTGCGA301.1468546E-7119.9641652
CCGGTAG600.0119.9641651
Produced by FastQC (version 0.11.5)