FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282392

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282392
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183279
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA202.192246E-6144.107591
ATTACGT306.8030204E-10143.989643
GCGTTGA151.2630752E-4143.989646
CGCTAGC151.2630752E-4143.989649
TGCGTTG151.2630752E-4143.989645
CGTGCAA100.007235865143.989627
ATCCGTA100.007235865143.989627
GTAGCTA100.007235865143.989626
CTACGTA100.007235865143.989624
CGTCGAA100.007235865143.989627
CCGAATG100.007235865143.989629
CGGTATA100.007235865143.989626
TAGTGCA100.007235865143.989626
ACGAGTG100.007235865143.989624
TACGTTA100.007235865143.989625
TACCTTA1050.0130.383071
GTTAGCA450.0127.990783
GCGTATA450.0127.990782
GCTGCGA403.45608E-11126.094141
ATCCGTG403.45608E-11125.990939
Produced by FastQC (version 0.11.5)