FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282404

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282404
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences197132
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGAGA253.8618964E-8143.965266
TCGTTAC202.2034783E-6143.965248
ATCTAAC100.0072399424143.965247
CATGCGA100.0072399424143.965242
CGTGCCA306.8030204E-10143.965243
GATCCGA151.2640716E-4143.965249
GCACGTC100.0072399424143.965247
AAGCGTA100.0072399424143.965247
GTAGGGC100.0072399424143.965246
TCTAGCG100.0072399424143.965249
CGGATTC151.2640716E-4143.965243
CGAGCTA100.0072399424143.965246
ACGTCGC100.0072399424143.965245
CGTAACG151.2640716E-4143.965247
TTACGCG202.2034783E-6143.965249
CACGTAA151.2640716E-4143.965248
GTAAGGG151.2640716E-4143.965248
GCGCTTA100.0072399424143.965249
TTCGCGA100.0072399424143.965249
CATTTCG100.0072399424143.965244
Produced by FastQC (version 0.11.5)