FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282424

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282424
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences223814
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGAAT151.2635128E-4143.987722
CGCGTTA306.8030204E-10143.987722
GTATTAG202.204877E-6143.955558
GGCGTGA100.0072420524143.955555
CCGTCGA100.0072420524143.955556
CTCGAAG100.0072420524143.955553
CCGAACA100.0072420524143.955553
CGGTCGG100.0072420524143.955556
AGCGTGC151.2646383E-4143.955545
AGTTTCG151.2646383E-4143.955544
TATATGA151.2646383E-4143.955549
TAGTTAC151.2646383E-4143.955546
TGCGTAC151.2646383E-4143.955545
GAGGCGA151.2646383E-4143.955547
GCGAGAA600.0131.988741
TCAGCGA600.0131.959248
CGGCTAG450.0127.9890751
CACCGAA450.0127.960488
CGCTTCA750.0124.789351
GCGTATG351.9899744E-9123.4180452
Produced by FastQC (version 0.11.5)