FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282427

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282427
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences283857
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGATA253.8675353E-8143.957722
TCCCGTA100.0072427415143.957724
TCGACTA253.8675353E-8143.957729
CGATTGA950.0128.89511
TTACGCG450.0127.962419
GGTATTG850.0127.1110841
CGGACGT403.45608E-11126.051821
CGTAAAT403.45608E-11125.9630057
AGCGTAT650.0121.896261
TGACTAG950.0121.313041
GTCGACT600.0119.9647758
CGCGAGA600.0119.9647752
TGTCGAC301.1480006E-7119.9647757
GGCTCGA850.0118.637011
TTGAACG550.0117.866641
CGGAGTC550.0117.866641
GCCGACA550.0117.783593
TCGCGAG1250.0115.247381
CTGATAG1000.0115.2473751
ATTACCT256.6926004E-6115.1661769
Produced by FastQC (version 0.11.5)