FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282431

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282431
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269712
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGACC100.007241286143.966087
GCGTTAG202.2049026E-6143.966083
ACCACGT400.0143.966089
GTTATGC100.007241286143.966088
AGCGTTA151.2645555E-4143.966065
GTCGAAA403.45608E-11125.9703148
CACTCGA403.45608E-11125.9703148
ACGCGTA351.9917934E-9123.422372
GTTAGTA351.9936124E-9123.399494
CGGACTG600.0120.060751
TGCGTGG850.0118.6482851
TGCGGGT501.8189894E-12115.258341
CCCATAC256.6654247E-6115.258341
CGGGTTG256.683893E-6115.194222
TTGATTG256.6900593E-6115.172869
GCGTTAC256.6900593E-6115.172866
GTATGAC501.8189894E-12115.172869
CTCGATC256.6900593E-6115.172863
GTCCAAC256.6900593E-6115.172868
AGTCGAA256.6900593E-6115.172867
Produced by FastQC (version 0.11.5)