FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282438

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282438
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences272850
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACGTA253.8706276E-8143.936059
CTCGAAC100.00724585143.936057
GCCAATA100.00724585143.936056
TACGTTA100.00724585143.936055
TAGTTGG850.0135.518891
GTCGACT750.0134.340328
TTGAACG700.0133.70391
CCGTACT1100.0130.898931
ACGCGAA500.0129.542439
CCTTACG950.0128.832091
TCGGTTG301.1489101E-7119.94675
ACGGTGA301.1489101E-7119.94675
AGCGTAT850.0118.5790251
CATCGAA850.0118.5790251
GCGTGAA550.0117.7658549
AACCGCG1000.0115.191051
CGATTCG256.6971425E-6115.1488344
AGGCGAC256.6971425E-6115.1488348
TGTTCGA256.6971425E-6115.1488349
GTCGTTA256.6971425E-6115.1488345
Produced by FastQC (version 0.11.5)