FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224471
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAA151.2637349E-4143.98155
ACGTTGA306.8030204E-10143.98152
TTTCGGC151.2637349E-4143.98156
ATTCGTA100.0072381627143.98154
TTCGTAA100.0072381627143.98155
CCGTACC100.0072381627143.98156
CCACGTA151.2637349E-4143.98154
CCTTACG750.0134.472611
TCGCGAG700.0133.786531
GCGCGAT500.0129.670031
AGCGTAT850.0127.1274641
TACACGA800.0126.068071
TAGTACG403.45608E-11125.983827
GCACCGA351.9917934E-9123.412737
GTACTCG351.9917934E-9123.412738
CCAAGCG351.9917934E-9123.412737
GCGTATC351.9917934E-9123.412732
TTGAACG650.0121.911981
GGCTCGA600.0120.0648351
AGCCGTA301.14609065E-7119.984594
Produced by FastQC (version 0.11.5)