FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282444

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282444
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228762
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGAAA100.0072400724143.96949
GTTAACG100.0072400724143.96949
AACGAGT202.2039258E-6143.96943
TTACGGA100.0072400724143.96949
TTCGCGA202.2039258E-6143.96949
ATACACG100.0072400724143.96945
ATTTCGT100.0072400724143.96943
TAATCCG100.0072400724143.96945
GTCGAAA253.8633516E-8143.969398
CGACGGA253.8633516E-8143.969399
CATGACG151.26419E-4143.969398
CACTCGA253.8633516E-8143.969398
CCTTACG650.0132.894821
AGCGTAT500.0129.572451
TCGGGAC500.0129.572451
CGTGATA403.45608E-11125.973237
TACGGAT750.0124.7734761
CGATTGA700.0123.402341
TATTAGC351.9917934E-9123.402349
TAGGACG351.9917934E-9123.402342
Produced by FastQC (version 0.11.5)