FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282447

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282447
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269936
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTACGA400.0143.987789
CGTCTAA151.2637973E-4143.987787
TGCGAGT100.007238027143.987784
CGTACGG202.2032527E-6143.987789
ACGTCTA151.2637973E-4143.987786
CGCGTAA100.007238027143.987785
CGTAACA450.0127.9891363
TAGTACG450.0127.9891367
GGCTCGA650.0121.903551
CGATTGA600.0120.056531
CGTAAAT301.1464181E-7119.9898157
TCTATCG301.1464181E-7119.9898158
ACGGAGA750.0115.254271
TTTAGCG256.685059E-6115.1902165
CGTGCCA256.685059E-6115.1902163
ATGCGAG256.685059E-6115.1902163
AGTCGTA501.8189894E-12115.1902168
GACCAAT950.0113.737771
GTCGTAA950.0113.737771
GTCGATA458.913048E-11111.990499
Produced by FastQC (version 0.11.5)