FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282450

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282450
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191924
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACGTAC100.0072376253143.979685
TAGACTA306.8030204E-10143.979687
TTAGCGT253.859168E-8143.979686
ACCGCTA100.0072376253143.979689
TGGCGAA202.2022286E-6143.979689
CGATTCG100.0072376253143.979684
TAACGCA151.2635156E-4143.979682
TGTGCGC151.2635156E-4143.979682
GCCATAG151.2635156E-4143.979686
TCGGATC100.0072376253143.979689
CTAGTAA253.859168E-8143.979685
TGCGACA100.0072376253143.979683
CGGGATA100.0072376253143.979682
AGCGAAC202.2022286E-6143.979689
TCATAGC151.2635156E-4143.979688
ATAACGC500.0129.615481
TTGAACG700.0123.443321
CGTACGT351.9899744E-9123.4111563
CGTCCGA351.9899744E-9123.4111567
TACGGTA650.0121.860711
Produced by FastQC (version 0.11.5)