FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences186598
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTTATGC151.261466E-4144.036741
CTCACGA100.0072346916143.998126
GCGGTGA100.0072346916143.998125
CGTCTAA100.0072346916143.998126
GCTTGAC100.0072346916143.998122
TCGGAGT151.2628145E-4143.998122
CCGTAGA151.2628145E-4143.998124
TTGCGAG100.0072346916143.998123
GCTCGAA100.0072346916143.998126
GCGTAAA100.0072346916143.998126
CCCGATA100.0072346916143.998122
AACACGA100.0072346916143.998126
CGTGCAA100.0072404933143.959537
ATGTCGA100.0072404933143.959539
TGTGCGA100.0072404933143.959537
TTCGGAA151.2641642E-4143.959537
CGATTGA550.0130.942491
ACGATAC550.0130.942491
CCGATTC403.45608E-11125.998363
CACGCGA403.45608E-11125.964598
Produced by FastQC (version 0.11.5)