FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183844
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGA100.0072351424143.994577
GTCGACT400.0143.994578
GACGTTG100.0072351424143.994572
CGATTCG151.2629094E-4143.994574
AGGCGAC100.0072351424143.994578
TATTGAG400.0143.994573
AAACGTA100.0072351424143.994574
CTAGTAA151.2629094E-4143.994575
GCGATAT202.2008462E-6143.994579
CGTACGA151.2629094E-4143.994577
AATAGTC100.0072351424143.994573
ATAGTCC151.2629094E-4143.994574
CGTCCTA100.0072351424143.994577
CAATCGA202.2008462E-6143.994578
TATAATG100.0072351424143.994579
TAATCGC100.0072351424143.994575
CGACCAA253.8562575E-8143.994552
TGTTACG550.0130.904147
AGCGATA500.0129.59518
CCTTCGA450.0128.031
Produced by FastQC (version 0.11.5)