FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194930
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGC500.0144.113251
CATGATA306.8030204E-10144.00232
AGCGACT253.8558937E-8144.00232
TTTCGCA151.2640462E-4143.965366
GCTATGC100.007239863143.965369
TCGGCTA100.007239863143.965368
ATGTACC100.007239863143.965366
CCGCGTA100.007239863143.965364
TTGCACG100.007239863143.965365
TAATCGC100.007239863143.965365
CATATTC750.0134.50571
TCGAATG403.45608E-11125.969698
CTCGAAA351.9772415E-9123.525651
TGTTACG351.9917934E-9123.398897
TCGCAAA600.0119.971138
TAGACTA301.14627255E-7119.971137
CGTTACG301.14627255E-7119.971139
TGGTTCG301.14627255E-7119.971134
AGACGAC301.14627255E-7119.971138
TCGTACC850.0118.6814961
Produced by FastQC (version 0.11.5)