FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences280581
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAT100.0072445134143.945659
ACGGGGT151.2653241E-4143.945653
CGTACGT151.2653241E-4143.945653
ACGTCGT500.0143.945653
ACGACTC306.82121E-10143.945659
TCGAATG650.0132.87298
GGCTCGA800.0126.064771
GATCGTA750.0124.864151
CGATTGA1050.0123.492021
AGTACGA351.9954314E-9123.381978
TTCGAAG1250.0121.022191
TAAACCG600.0120.06171
CGCTATC600.0120.06171
TAGCGTA301.1485463E-7119.9547049
TGCGTGG850.0118.64921
TCGTTAC550.0117.7737058
ACGAAAC550.0117.7737059
TACGGAT750.0115.259221
CTCGAAT256.6952543E-6115.156527
CCGCATA501.8189894E-12115.156529
Produced by FastQC (version 0.11.5)