FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences196621
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAG100.0072375336143.981193
CATGCGA100.0072375336143.981192
CACGCGA100.0072375336143.981198
ATTCGCT100.0072375336143.981192
AAACGTA100.0072375336143.981194
TCGCTTA151.2635095E-4143.981198
CGTAAAT100.0072375336143.981197
TTACGAG151.2635095E-4143.981193
TAGGACG151.2635095E-4143.981192
GGTTACA151.2635095E-4143.981197
GCGTATA100.0072375336143.981192
ATCGATC100.0072375336143.981197
CCCGATA151.2635095E-4143.981192
GCGGTAT253.8593498E-8143.981172
TCGACTA253.8593498E-8143.981179
ACACGAA450.0127.983287
CGATTGA403.45608E-11126.015581
TCGGAAA403.45608E-11125.9835368
GGCTCGA351.9863364E-9123.4438251
GGTACCG351.9899744E-9123.412447
Produced by FastQC (version 0.11.5)