FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences234047
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATA202.2059357E-6143.944449
CGCGAGA151.2650997E-4143.944442
GTTAACG253.8675353E-8143.944449
TTCGGAA202.2059357E-6143.944447
CGATTGA600.0132.09021
TCTAACT550.0130.858588
GTACGTA1000.0129.688551
CGCTAGC500.0129.559
GATCGTA403.45608E-11126.086091
TTACGGA403.45608E-11125.9513859
TAAACCG750.0124.885271
CATATAC301.1480006E-7119.9537058
GTTATGC301.1480006E-7119.9537058
TTGAACG550.0117.898691
TAGTTGG1050.0116.651081
TTACGAG256.6931916E-6115.1555563
CAATAGC501.8189894E-12115.1555568
CTAATCG256.6931916E-6115.1555564
TACGTAT501.8189894E-12115.1555562
ATCTAAC700.0113.0992057
Produced by FastQC (version 0.11.5)