FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences224715
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAAGCG100.007243149143.948385
CGATTCG100.007243149143.948384
TAGTCGA100.007243149143.948386
GCGTAAA306.82121E-10143.948386
TCGCGAA100.007243149143.948389
ATTACGA450.0127.954129
CGAAGTG351.9936124E-9123.384335
GATCGTA650.0121.8295751
TGTTACG301.147655E-7119.956987
CGTACGT301.147655E-7119.956983
CTTTGCG550.0117.8021551
TTGCCGT550.0117.8021551
TTCGCGA550.0117.775939
GATCGCG750.0115.184331
AGCGTAT501.8189894E-12115.1843261
ACAACGC256.6917E-6115.158699
CAACGAT501.8189894E-12115.158698
CATTACG501.8189894E-12115.158698
ACTAGTC501.8189894E-12115.158693
TGCGAAA256.6917E-6115.158699
Produced by FastQC (version 0.11.5)