FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences267635
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTGC253.864443E-8143.973282
TGCGAAA151.2642921E-4143.973289
TTAGTTG100.007240172143.973286
GTGCGTA100.007240172143.973282
TACGGAT1100.0130.95821
TCGTAGG450.0127.976258
CGGACTG403.45608E-11126.047271
CGATTGA1100.0124.410291
ACGATAC1100.0124.410291
TGACTAG650.0121.891871
CGTAAAT301.1470911E-7119.977747
TTGCACG301.1470911E-7119.977745
GCCGTAG301.1470911E-7119.977743
TAAACCG1050.0116.6151661
TAGTTGG501.8189894E-12115.243221
GAAGCGT256.6882985E-6115.178636
GTTAAGA750.0115.178638
GGTTACG256.6882985E-6115.178637
CGATAGT256.6882985E-6115.178634
CGATAGG256.6882985E-6115.178634
Produced by FastQC (version 0.11.5)