FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282493

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282493
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences273045
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACTA202.2041822E-6143.97629
CTAATGG950.0136.423481
AGCGATA500.0129.578578
CGCCGAC403.45608E-11126.002242
GCGATAT403.45608E-11125.9791649
CGTTAGA1100.0124.365861
ATTACGT351.9936124E-9123.408163
TAACACG351.9936124E-9123.408165
CCTTACG1250.0120.962141
GTCTAGT600.0119.980168
ATAACGC800.0117.002081
CTATTAA256.681781E-6115.202062
CTTACGG501.8189894E-12115.202062
CGAAAGA256.687871E-6115.1809544
AGTGACG256.687871E-6115.1809547
CGATCGG900.0112.0021
GTACGAA458.913048E-11111.981488
CGTCTAC650.0110.771211
CGGCTAG1500.0110.401971
GCGCCAA850.0110.119611
Produced by FastQC (version 0.11.5)