FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282499

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282499
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147675
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACCAAT550.0144.051511
TTTATGC550.0144.051511
CGAACAT253.8433427E-8144.05151
GCGAGAC100.007239815143.953955
TTTAGCG100.007239815143.953955
AACGTCA151.2638293E-4143.953955
TAATAGA151.2638293E-4143.953954
GCGACTA151.2638293E-4143.953952
CTACTCG100.007239815143.953954
GGTCGGA100.007239815143.953956
CGAGTAC151.2638293E-4143.953955
CTATCCA100.007239815143.953955
AGGACGA100.007239815143.953958
ACTTCGA100.007239815143.953959
TTCGTTA100.007239815143.953957
GATTTCG100.007239815143.953952
GTCTAGG202.202487E-6143.953958
TAACGCC100.007239815143.953952
TATGCCA202.202487E-6143.953953
GCGTTAG100.007239815143.953956
Produced by FastQC (version 0.11.5)