FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144652
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGGC306.7848305E-10144.02041
TAGTTGG253.8478902E-8144.02041
CGGACGT253.8478902E-8144.02041
GTGTTAC151.2631937E-4143.970632
AGGTATA151.2631937E-4143.970636
TGTCACG100.0072371573143.970635
CTAGAAG202.2010645E-6143.970636
AGGTAGC151.2631937E-4143.970638
GTCGCCT100.0072371573143.970637
ATTAGTC100.0072371573143.970634
ATAGGAA100.0072371573143.970636
ACCGCGA151.2631937E-4143.970632
GACGTTG151.2631937E-4143.970632
CGTTGGT100.0072371573143.970637
TTAGAAC151.2631937E-4143.970639
GACCGGA100.0072371573143.970639
TAACGCG151.2631937E-4143.970639
TGAAACG100.0072371573143.970632
CGACACA202.2010645E-6143.970635
TATGCCA202.2010645E-6143.970633
Produced by FastQC (version 0.11.5)