FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282512

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282512
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences226482
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA306.7848305E-10144.043521
GTCGTAA351.2732926E-11144.043521
GGTGCGT306.7848305E-10144.043521
GTCGAAT100.0072432263143.948127
CTAAGCG100.0072432263143.948125
TATGCAG100.0072432263143.948123
CCGTAGC100.0072432263143.948126
TTAAGTA100.0072432263143.948129
AATCTAG100.0072432263143.948126
ATAAGTC351.2732926E-11143.948123
GCGATAT351.2732926E-11143.948129
ATGCGAG100.0072432263143.948123
ACTTTTA100.0072432263143.948123
TAAACGC151.2649181E-4143.94819
AGGCGAC151.2649181E-4143.94818
GCGTTGC151.2649181E-4143.94819
GTTCGAG151.2649181E-4143.94816
ACTACAC253.8666258E-8143.94812
GCGGACA151.2649181E-4143.94813
ATTGCGT151.2649181E-4143.94813
Produced by FastQC (version 0.11.5)