FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282515

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282515
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215801
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTAG650.0144.078141
CATCGAA850.0144.078121
GGACGCA100.007233485144.011352
ATCGAAA100.007233485144.011352
CGGAAAT100.0072384994143.977989
CCGATAG202.2029872E-6143.977983
GTCCTAC100.0072384994143.977987
CGCATGG151.2637912E-4143.977989
TTATCGG100.0072384994143.977989
ATCGTGC100.0072384994143.977985
AGCGGAT151.2637912E-4143.977988
CGCACGA202.2029872E-6143.977986
CTTTACG151.2637912E-4143.977989
CGCAAGG100.0072384994143.977986
CCAAACG151.2637912E-4143.977987
AAGTCGT202.2029872E-6143.977987
CCTTACG700.0133.786831
GACACGC700.0133.786831
TAGTTGG600.0132.071621
AGTACGA500.0129.580188
Produced by FastQC (version 0.11.5)