FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219921
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTATC306.8030204E-10144.019561
GCGACTG253.8598955E-8143.986822
GACGTCG151.2635144E-4143.986822
CGTACCT151.2635144E-4143.986822
CGAGAAT100.0072372654143.986822
CGGTAGG100.0072372654143.986822
ATAGGTC151.26466E-4143.954074
CGCATTG100.0072421883143.954076
TACGAAA100.0072421883143.954079
CACCGAA306.82121E-10143.954078
ACGAGTA100.0072421883143.954074
TACGTTG100.0072421883143.954075
CCTTACG700.0133.732451
TGACTAG650.0132.941131
TATTGAG550.0130.867323
CGGGTAG450.0127.959173
GCGCGAT850.0127.076091
TACACGA800.0126.017121
TTAAGCG403.45608E-11125.988462
CGGAGTA403.45608E-11125.959814
Produced by FastQC (version 0.11.5)