FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282522

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282522
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217219
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGAAGCG202.2023942E-6143.986195
GTCCTAC100.007237299143.986197
AGGCGTA202.2023942E-6143.986194
TATGCTA202.2023942E-6143.986192
TAGTTGC202.2023942E-6143.986197
AGCGAAC202.2023942E-6143.986199
TCGAGAG100.007237299143.986198
TTCGCTA100.007237299143.986197
CTGGCGA100.007237299143.986199
CCCATAC202.2023942E-6143.986191
GTACGAT202.2023942E-6143.986199
TTTAGAC151.2635156E-4143.986185
TTCTAAC450.0143.986187
GGTCGTA253.8598955E-8143.986184
GGATGCG151.2635156E-4143.986189
AGTATGG253.8598955E-8143.986187
ACTAGTC351.2732926E-11143.986183
TGCGAGA306.8030204E-10143.986184
TTGACGT253.8598955E-8143.986189
CTCGCGT253.8598955E-8143.986183
Produced by FastQC (version 0.11.5)