FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282525

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282525
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174729
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGGAG600.0144.141561
CATATTC253.831883E-8144.141541
GCGCGAT500.0144.141541
TAATAGG151.2634331E-4143.976534
ACCGGAA100.0072375475143.976539
GTATTGT151.2634331E-4143.976532
GGATGCG100.0072375475143.976539
TAGACGC202.2019103E-6143.976537
CGTTGTG100.0072375475143.976537
GCAAACG100.0072375475143.976537
ACTTACC151.2634331E-4143.976532
CGTTAGG100.0072375475143.976537
AGACGAC151.2634331E-4143.976538
CCGTAGC100.0072375475143.976536
CATAGTG351.2732926E-11143.976535
GTTCGAG100.0072375475143.976536
TGCGATA100.0072375475143.976539
GTATGGT100.0072375475143.976538
TGCGAAG151.2634331E-4143.976533
GGTATAG100.0072375475143.976537
Produced by FastQC (version 0.11.5)