FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences170230
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTACTTA253.8549842E-8143.99479
GTCGAAC100.007234653143.99478
ACGTTGA202.2003624E-6143.99472
CTATCCC100.007234653143.99479
CTATCCA100.007234653143.99475
CGTGATA100.007234653143.99477
CGTTGAG202.2003624E-6143.99473
GACCGGA202.2003624E-6143.99479
ATAGCTA151.262741E-4143.99476
TTGTCGA100.007234653143.99476
CGACCAA202.2003624E-6143.99472
TATTGAG351.2732926E-11143.99473
CGGGGTA100.007234653143.99474
TGCATAT306.8030204E-10143.99472
GTTCGTG151.262741E-4143.99478
GTTAACG253.8549842E-8143.99479
CGGATTC100.007234653143.99473
CGAGGAA100.007234653143.99472
ACTACAC151.262741E-4143.99472
TCGTAAA100.007234653143.99472
Produced by FastQC (version 0.11.5)