FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282538

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282538
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146699
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTCG151.2605122E-4144.048432
CGACCAA151.2605122E-4144.048432
GACCAAT306.7666406E-10144.048431
TACCTTA500.0144.048431
ACCTTAA151.2605122E-4144.048432
CCACCTA351.2732926E-11144.048431
CGCTTCA253.8437065E-8144.048431
CAACGAG100.0072255754144.048422
TATGCTA202.1952546E-6144.048422
CGCTATC202.1952546E-6144.048421
TATTCTA100.0072255754144.048422
ACGGATG100.0072255754144.048422
AAACCGA202.1952546E-6144.048422
ACGGTAC202.1952546E-6144.048422
GATACGT151.2622264E-4143.999313
TCGTTAC202.1989854E-6143.999318
GCGAAGT151.2622264E-4143.999314
CCCTAAG100.007232948143.999313
TAGGTCG100.007232948143.999317
GGCAATG151.2622264E-4143.999316
Produced by FastQC (version 0.11.5)