FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282541

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282541
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149722
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGATTC100.007240523143.949899
CGGGTTA100.007240523143.949892
GATACGT100.007240523143.949893
CCTTACG351.2732926E-11143.949891
CCTTAAT100.007240523143.949899
GCGACTT100.007240523143.949892
GTCGAAA202.2028962E-6143.949898
TTTAAGC202.2028962E-6143.949899
CTATAGA100.007240523143.949894
CGATTGA450.0143.949891
CCTAATC151.2640057E-4143.949893
CGTTCTA100.007240523143.949895
CCGCTCA100.007240523143.949894
TCGGCAA100.007240523143.949894
ATCGTAC253.8597136E-8143.949892
GTAATAG151.2640057E-4143.949898
GCCTGTA151.2640057E-4143.949894
TATTAAG202.2028962E-6143.949893
GGACGAA306.8030204E-10143.949899
ATTGGCG151.2640057E-4143.949899
Produced by FastQC (version 0.11.5)