FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences205117
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGC202.2023287E-6143.983188
AGCCGAC151.2635186E-4143.983182
TCGCACA151.2635186E-4143.983185
GTCGAAA151.2635186E-4143.983188
CTTCCGA100.0072374595143.983186
CATGACG151.2635186E-4143.983188
TAGGTCG100.0072374595143.983185
GGCGTGA100.0072374595143.983185
CGTTGCA100.0072374595143.983183
GGATCGC100.0072374595143.983189
TTCCGCG202.2023287E-6143.983187
CTGCGAC151.2635186E-4143.983189
TCGCTAG100.0072374595143.983188
TCAGACG100.0072374595143.983187
CGGGATC151.2635186E-4143.983183
CTCGCCA202.2023287E-6143.983183
TTAGTTG100.0072374595143.983185
ACGTCGG151.2635186E-4143.983185
CGTAACG151.2635186E-4143.983187
TCTTTCG151.2635186E-4143.983184
Produced by FastQC (version 0.11.5)