FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences207908
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGAGCC550.0144.044771
TCGTTGC151.263813E-4143.975488
GCGACTA253.860987E-8143.975482
CGTATGA306.8030204E-10143.975487
TACCATA151.263813E-4143.975485
TCCGATA151.263813E-4143.975488
GGCGTAC450.0143.975489
TCGTGAC306.8030204E-10143.975488
TAGGATG151.263813E-4143.975487
GCCGTAA151.263813E-4143.975485
GTTATGC253.860987E-8143.975488
TACGTTA253.860987E-8143.975485
GTACGAA253.860987E-8143.975488
AACGTTA100.0072386893143.975465
CTTCGAC100.0072386893143.975462
AGATAGC100.0072386893143.975468
CGCGGAT400.0143.975469
TTAGCGA100.0072386893143.975466
GCGAAGG100.0072386893143.975464
TAGCGTA100.0072386893143.975469
Produced by FastQC (version 0.11.5)