FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282554

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282554
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences199542
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGAT650.0144.062451
CCCATAC253.846617E-8144.062451
CGCTTCA500.0144.062451
AGCGTGC100.0072416794143.954135
GGTGTAC202.2043914E-6143.954135
GTCGAAC151.2644831E-4143.954138
ACGTTAA202.2043914E-6143.954136
GTCCTTA100.0072416794143.954137
CGTGACT100.0072416794143.954137
AAGACGC100.0072416794143.954137
GTTAGTA100.0072416794143.954134
TTCCGGT100.0072416794143.954133
CGTTCTA100.0072416794143.954135
TGTGCGC100.0072416794143.954132
CTGCGTA151.2644831E-4143.954139
GTTACAA306.82121E-10143.954134
ACCTATA202.2043914E-6143.954132
TCGGAGT202.2043914E-6143.954132
AGCATGC202.2043914E-6143.954136
GGGCGTA151.2644831E-4143.954132
Produced by FastQC (version 0.11.5)