FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282560

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282560
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences260995
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCGAT750.0143.991941
CTAGATA100.0072414097143.964376
CGGAATC100.0072414097143.964379
TCGTTAG100.0072414097143.964376
GTCGAAA100.0072414097143.964378
CTATTCG100.0072414097143.964374
GCGTGCA100.0072414097143.964376
CACGTGA202.204899E-6143.964378
TTCGACC100.0072414097143.964377
TAAAGCG253.8655344E-8143.964365
GGATGCG151.2645689E-4143.964369
CGGTTGG151.2645689E-4143.964366
ATTACCG151.2645689E-4143.964362
AGTATTG306.82121E-10143.964368
TCCGTAA151.2645689E-4143.964365
GCGGAGT151.2645689E-4143.964363
GACGACG151.2645689E-4143.964369
CGTCCTA253.8655344E-8143.964366
TTTTCGG151.2645689E-4143.964365
GTACGTA750.0134.392491
Produced by FastQC (version 0.11.5)