FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282563

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282563
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences248187
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATAGG253.8598955E-8143.996382
ACGCGTG450.0143.996382
GCGACTA100.007236386143.996372
CGCGATA202.202245E-6143.996372
TAATGGC151.2633749E-4143.996372
CGTATCG100.0072407476143.967364
CCCGTGA100.0072407476143.967365
CTAACGG100.0072407476143.967369
CGAATGC253.864625E-8143.967369
CGAGACG100.0072407476143.967366
GGCGCTC202.204455E-6143.967369
CCGTTCG100.0072407476143.967364
TCACGCG151.2643897E-4143.967358
TCCGATA306.82121E-10143.967358
GTATCGT151.2643897E-4143.967355
CGAAGGA500.0129.570625
GGCTCGA1250.0126.7934651
CGTACCT403.45608E-11125.996822
CGCTATC750.0124.872361
TCGTAGG750.0124.771718
Produced by FastQC (version 0.11.5)