FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282564

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282564
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences214293
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATATG306.82121E-10143.957068
GGTCGAG151.2645102E-4143.957067
GTCCTTA151.2645102E-4143.957067
TTGTATA151.2645102E-4143.957066
ATTCGAC306.82121E-10143.957069
CACGATC151.2645102E-4143.957063
GCGTTGC151.2645102E-4143.957069
TGCGAAG151.2645102E-4143.957063
ACGTACC151.2645102E-4143.957066
GCGACTA202.2045442E-6143.957052
TTCGTAG202.2045442E-6143.957057
CGTCTAA202.2045442E-6143.957057
TGCGGAC100.007241611143.957052
TATGCAG202.2045442E-6143.957053
GTTCGTC202.2045442E-6143.957058
AAACCGG400.0143.957052
GATATGC100.007241611143.957059
AGCGATA202.2045442E-6143.957058
CGTACGA202.2045442E-6143.957057
ACTCGAA100.007241611143.957056
Produced by FastQC (version 0.11.5)