FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282567

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282567
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences255260
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAATCGA100.007234929144.006875
ACTTCGC100.007234929144.006875
GCCGCAG202.2015665E-6144.006873
GCCGACA253.8586222E-8144.006873
TGCGAGA202.2015665E-6144.006874
TCGTAGC202.2015665E-6144.006876
CGCGTAA100.007234929144.006875
CGCCGAT100.007234929144.006872
TTACACG100.007234929144.006875
GTGCGCA100.007234929144.006873
TTTTCGA202.2015665E-6144.006875
TAGTAGA151.2630502E-4144.006856
TTCCGCG351.2732926E-11143.950447
GTTACCG202.2058666E-6143.950448
CTTGACG100.007243411143.950449
CGTAACG151.2650237E-4143.950447
TTACGCG151.2650237E-4143.950449
CCGAACG202.2058666E-6143.950449
CCGCATA202.2058666E-6143.950449
CGATCGG950.0136.427551
Produced by FastQC (version 0.11.5)