FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282577

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282577
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences290117
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAA100.007242401143.960535
ATTACGA202.2056101E-6143.960539
GCCGATA202.2056101E-6143.960532
CTAGTAA151.2648471E-4143.960536
CGGTGAC100.007242401143.960536
TTTTCGG202.2056101E-6143.960535
CGAAGGA151.2648471E-4143.960535
ACGAGCC500.0129.609161
GGTATTG900.0128.009051
GCGCTTA450.0127.964919
TGACTAG1200.0126.0088961
CGGACGT800.0126.0088961
TGCGGGT750.0124.8088151
ACGAGGA750.0124.8088151
TCCCGAT700.0123.437281
GAAGCGT351.9954314E-9123.394746
CGTACGA351.9954314E-9123.394747
CTTCGCA650.0121.812753
GTCCGAA650.0121.812758
CCTTACG600.0120.008481
Produced by FastQC (version 0.11.5)