FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282580

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282580
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences295976
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACGGC450.0144.030081
TGAAACG253.8602593E-8144.005742
TGCGCAA100.007239333143.981414
ATGCGAG306.82121E-10143.981413
ACCGTAG151.2641419E-4143.981415
GATGCGA100.007242991143.957099
ATCGACG100.007242991143.957097
TCGTGGA151.2649932E-4143.957088
GGTATTG950.0136.449551
AGCGAAC800.0134.959789
GCGCGAT750.0134.428091
CGGACTG1200.0132.027591
TAGCCGG900.0128.026731
GAACATA450.0128.005112
CGTCTAC850.0127.0853651
TGAACGA351.9899744E-9123.4334952
TACTTAG351.9954314E-9123.3917859
TGACTAG1000.0122.425581
TCCCGAT650.0121.871611
ACGAGTA650.0121.830434
Produced by FastQC (version 0.11.5)