FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282586

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282586
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203502
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGACTAG550.0144.175661
CCTTCGA151.2567968E-4144.175641
CGTTGGG306.7484507E-10144.175641
CGATTGA850.0144.175641
CCGGGAT450.0144.175631
CATGCGG202.198427E-6144.033922
TTATGCA202.198427E-6144.033922
CGGGATA202.198427E-6144.033922
TGATCGA100.007235112143.998545
GTCGCGA151.2629683E-4143.998548
ACCGGAA306.8030204E-10143.998549
CGTATAG100.007235112143.998543
GAAGACG100.007235112143.998546
CGTGCTC100.007235112143.998543
AACTTCG100.007235112143.998544
AAGGTAC100.007235112143.998545
CGATTCC100.007235112143.998544
GCGTTGG100.007235112143.998546
ACCTCGA100.007235112143.998545
AGTATCG100.007235112143.998548
Produced by FastQC (version 0.11.5)