FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282587

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282587
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences203502
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC98124.821574235142652No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC78863.875146190209433No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC55532.7287201108588612No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC45732.247152362138947No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA15510.7621546716985583No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG10600.520879401676642No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA7480.36756395514540396No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG6980.3429941720474492No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT6100.2997513537950487No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG5100.2506117875991391No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGC5020.2466806223034663No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATG4640.22800758714902064No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATG4520.22211083920551147No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT4360.21424850861416597No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCC4350.21375711295220684No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTC4110.20196361706518853No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTAAA4080.20048943007931128No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATTC3970.19508407779776119No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC3920.19262709948796572No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTTT3810.18722174720641566No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTTTC3790.18623895588249748No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATGG3780.18574756022053837No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAAAG3780.18574756022053837No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGAACTC3700.1818163949248656No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGTAAA3690.18132499926290652No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGA3690.18132499926290652No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTTTG3600.17690243830527463No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG3590.17641104264331553No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTCTT3570.17542825131939735No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTG3530.17346266867156096No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGCTTC3510.17247987734764278No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTGT3480.1710056903617655No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG3350.16461754675629725No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGATG3210.1577380074888699No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGGAGA3150.15478963351711533No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTGG2910.142996137630097No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGACTG2770.13611659836266965No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT2770.13611659836266965No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGCTTT2690.13218543306699687No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCAGC2680.1316940374050378No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCAATC2540.12481449813761043No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC2220.10908983695491936No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTCGGATTT2210.10859844129296027No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCCGG2180.10712425430708299No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTCTA2070.10171890202553292No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGATG17500.0129.244481
GTCTCTC25050.0118.731772
AGTCTCT25350.0117.442391
TCAGCTG25550.0117.2207647
AGCTGGT25700.0116.53669
CAGCTGG26650.0112.9226768
TCTCTCA26550.0112.7283863
CTCAGCT27950.0107.412876
CTCTCAG28000.0106.890664
TCTCAGC28300.0106.364995
GGCTCAA30950.073.041757
CTCAAAC31300.072.6850059
GCTCAAA31300.072.6850058
TGGCTCA31250.072.340546
ATGGCTC32350.070.343125
CTGATGG32700.069.320132
GATGGCT33150.068.5293664
TGATGGC37250.060.9865383
TCAAACA32250.057.8192149
AGGCAGT1255.2700465E-428.8760282
Produced by FastQC (version 0.11.5)