FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282589

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282589
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208597
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGAAG253.8597136E-8143.983953
TGATCCC100.007237432143.983935
CGGGTAC151.2635223E-4143.983935
CGTGGCA100.007237432143.983933
TGCCAAT151.2635223E-4143.983935
GGGTCGT202.2023578E-6143.983933
GGCCGTC100.007237432143.983932
GCGTTAC151.2635223E-4143.983936
TAGTTTA100.007237432143.983936
CTGCGGA306.8030204E-10143.983931
GCCGAGT351.2732926E-11143.983933
TACGAGA151.2635223E-4143.983934
CCGAGAA100.007237432143.983935
CATTTCG100.007237432143.983934
TTACATG100.007237432143.983935
GCGTACT100.007237432143.983936
TAACTTA100.007237432143.983932
GATAGCG100.007237432143.983935
TAGTAGA202.2023578E-6143.983936
ACGGTCG100.007237432143.983934
Produced by FastQC (version 0.11.5)