FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282599

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282599
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265372
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTC147015.539770586195981No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCC116154.376874726798608No Hit
TCTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTC78072.9419079631611473No Hit
CTGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCC63542.3943746891156565No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTA33521.2631325083279321No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAATG14600.550171080596295No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTA13090.49326982500037686No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTTT9790.36891608760532385No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCAG8980.3383928975174472No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTTGC8230.310130684473117No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTTTT7360.2773465173416939No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTAAA7150.2694330976892814No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCTGG7130.268679438674766No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGCCTG7060.2660416321239618No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTCTT6820.25699772394977616No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGTATG6610.24908430429736372No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGAGTTCC6490.24456235021027084No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATACGGATGA6450.24305503218123992No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATGG6420.2419245436594667No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATGGGGTTTG6370.240040396123178No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTATG6340.23890990760140485No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTTC6040.22760502238367272No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCT5830.2196916027312603No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGCTTC5700.2147928191369097No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGAAAG5640.2125318420933633No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGTTTC5610.21140135357159007No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACGTTTTTCAGGTCCTC5550.20914037652804365No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGTTG5530.2083867175135282No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGAACTC5500.20725622899175497No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGATTC5360.20198061589014665No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGTAAA5240.19745866180305383No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCGAGCGTG5230.19708183229579607No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGGATG4770.17974767496194022No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTGT4570.1722110848167855No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTGGGGAGA4520.17032693728049683No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGACTG4370.16467449467163076No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCTGG4360.16429766516437302No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGCTTT3860.14545618980148622No Hit
GTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCTTTAA3700.13942691768536242No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATATGGGTTT3680.13867325867084696No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGCAATC3580.1349049635982696No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTTGGCCGG3450.13000618000391903No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGAATA3400.12812203246763035No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCAGGATTC3280.1236000783805375No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATAGGGCAGC3170.11945495380070242No Hit
GATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCTC3060.1153098292208673No Hit
TGATGGCTCAAACACAGCGACCTCGGGTGGGAACACCTTGTTCAGGTCCT2950.1111647046410322No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGAGAG2940.11078787513377447No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATCTGGTCTA2930.11041104562651673No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTAAGATCC2850.10739640956845485No Hit
AGTCTCTCAGCTGGTACACGGCAGGGTCAGGGTTCTGGATATTCGGATTT2770.10438177351039296No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGGC151.2504513E-4144.371252
TACGGCG100.0072334884144.017536
ACGGCGA100.0072375652143.990397
TCTGATG25850.0126.2916951
GTCTCTC36750.0122.371822
TCAGCTG37300.0120.442377
AGTCTCT37600.0119.865361
AGCTGGT37750.0119.197359
TCTCTCA38200.0117.637913
CAGCTGG39350.0115.0825358
CTCAGCT39050.0115.06656
CTCTCAG39900.0112.562024
TCTCAGC40650.0110.3810965
GGCTCAA44150.074.522777
CTCAAAC44500.073.774869
GCTCAAA44800.073.441538
CTGATGG44800.073.15242
GATGGCT45250.072.8072054
TGGCTCA45750.072.0874566
ATGGCTC47100.070.49325
Produced by FastQC (version 0.11.5)