FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282608

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282608
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences204148
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGATTGA600.0144.050741
ACTGCGA100.007237887143.980162
AACGGAT100.007237887143.980162
ATGGCGC151.2636154E-4143.980152
CCTTCCG202.205219E-6143.944895
TGTCACG100.007243192143.944895
ATAGGAT100.007243192143.944899
GTTAGTA100.007243192143.944894
ACCCTAG202.205219E-6143.944893
AAAGTTA100.007243192143.944896
AGAGTTG202.205219E-6143.944898
TAGTTGC100.007243192143.944897
AGACGAC202.205219E-6143.944898
CGAGGTG100.007243192143.944896
ACGTGTG100.007243192143.944894
AACGCAC100.007243192143.944899
TTCGGAA100.007243192143.944897
TTTGACA202.205219E-6143.944899
GACGACG202.205219E-6143.944899
TCGACTG202.205219E-6143.944899
Produced by FastQC (version 0.11.5)