FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282611

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282611
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences194726
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGAAC202.2016247E-6143.988718
TAATAGC151.263228E-4143.988719
GTATTGA202.2016247E-6143.988712
TCCGACG400.0143.988719
CGTGATG306.8030204E-10143.988717
ATTGTCG100.00723635143.988714
GTAGGTA100.00723635143.988714
CGATTCC151.263228E-4143.988714
TAACGCA100.00723635143.988712
TCGATCC151.263228E-4143.988718
GCGTTAA151.263228E-4143.988716
GCCGCAC202.2016247E-6143.988714
TAGTTGC100.00723635143.988717
TACATAG151.263228E-4143.988716
AGGCCCG100.00723635143.988718
CCATCGA202.2016247E-6143.988717
TAACATC100.00723635143.988715
GAGCGCC100.00723635143.988717
CGAGGAA151.263228E-4143.988712
CGGGCTA151.263228E-4143.988712
Produced by FastQC (version 0.11.5)