FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005282625

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005282625
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences202475
Sequences flagged as poor quality0
Sequence length150
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCGAT306.8030204E-10144.014591
TAAACCG650.0144.014571
TTGGTTA151.2648846E-4143.943452
ACTCGTG151.2648846E-4143.943454
TACTCTA151.2648846E-4143.943455
GCTACCG306.82121E-10143.943459
CGGGTAC100.0072433664143.943445
CGTGGCA202.2052864E-6143.943443
TAGGTTC100.0072433664143.943445
ACCGATA100.0072433664143.943449
GCACCGA100.0072433664143.943447
TTGAGAA100.0072433664143.943445
GTAGAAT100.0072433664143.943446
TACGAAA100.0072433664143.943449
TGCGAGA100.0072433664143.943444
CCGAGTA100.0072433664143.943444
GTATCTA100.0072433664143.943445
ATGACGG253.8657163E-8143.943449
CGTACGA100.0072433664143.943447
TACAGCG100.0072433664143.943446
Produced by FastQC (version 0.11.5)